CHARRING METHOD FOR DETERMINATION OF LIPIDS
This assay is quantitative and rapid
and enables to get reliable results with acylglycerols as well as other lipids,
including waxes, sterols and long-chain bases, the lipids being converted to
carbon by charring in concentrated sulfuric acid. The limit of sensitivity is
about 2-5 mg
according to the lipid. The linearity of the response is observed for all lipids
up to about 100 mg
but the slopes differ for different lipids. Under precise conditions, the
optical density of the reaction mixture appears directly proportional to the
carbon content of the lipid under assay (Marsh JB et al., J Lipid Res 1966,
In glass tubes containing lipid samples thoroughly dried or scraped silica gel spots, add 1 ml of concentrated sulfuric acid.
Heat the tubes at 200°C for 15 min in a sand bath (or a heating bloc).
Cool rapidly the tubes (in a cold metal block or in water) during 2 min and transfer the tubes in an ice bath.
Add 1.5 ml water , vortex thoroughly and replace in the ice for 5 min.
Remove the tubes from the ice and left standing for 10 min.
If silica gel is present, centrifuge the tubes at low speed for 5 min.
Transfer the reaction mixture in optical cuvettes and measure the optical density at 375 nm. A solvent blank is included in the assay together with tubes containing known amounts of standard lipids.
An alternate procedure has been evaluated using sulfuric acid, anisaldehyde or vanillin (Johnsson R et al., J Chromatogr A 2007, 1164, 298). Practical conditions have been compared before scanning the TLC plates.